TY - JOUR
T1 - Waste activated sludge hydrolysis during ultrasonication
T2 - Two-step disintegration
AU - Cho, Si Kyung
AU - Shin, Hang Sik
AU - Kim, Dong Hoon
PY - 2012/10
Y1 - 2012/10
N2 - In order to clearly describe the hydrolysis of waste activated sludge (WAS) during ultrasonication by a 2-step disintegration process, concentrations of ribonucleic acid (RNA) and bound extracellular polymeric substance (EPS) were measured. Apparently, different decreasing patterns of RNA and EPS concentrations during WAS hydrolysis made it possible to distinguish the floc disintegration (FD) and cell lysis (CL). Initially, FD and CL appear to take simultaneously, but the dominant hydrolytic process is shifted from FD to CL after 10. min of ultrasonication. Additional kinetic analysis of WAS hydrolysis was also conducted. A five-fold greater hydrolysis rate constant of FD relative to that of CL was observed, reflecting the different strengths of floc and cells. Therefore, different rates of increased solubilization during WAS hydrolysis appear to account for the initial disintegration of the rather loose part (sludge floc) and the subsequent disintegration of the rigid part (microbial cells).
AB - In order to clearly describe the hydrolysis of waste activated sludge (WAS) during ultrasonication by a 2-step disintegration process, concentrations of ribonucleic acid (RNA) and bound extracellular polymeric substance (EPS) were measured. Apparently, different decreasing patterns of RNA and EPS concentrations during WAS hydrolysis made it possible to distinguish the floc disintegration (FD) and cell lysis (CL). Initially, FD and CL appear to take simultaneously, but the dominant hydrolytic process is shifted from FD to CL after 10. min of ultrasonication. Additional kinetic analysis of WAS hydrolysis was also conducted. A five-fold greater hydrolysis rate constant of FD relative to that of CL was observed, reflecting the different strengths of floc and cells. Therefore, different rates of increased solubilization during WAS hydrolysis appear to account for the initial disintegration of the rather loose part (sludge floc) and the subsequent disintegration of the rigid part (microbial cells).
KW - 2-Step disintegration process
KW - First order kinetic analysis
KW - Hydrolysis
KW - Ultrasonication
KW - Waste activated sludge (WAS)
UR - http://www.scopus.com/inward/record.url?scp=84865546256&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2012.07.024
DO - 10.1016/j.biortech.2012.07.024
M3 - Article
C2 - 22850171
AN - SCOPUS:84865546256
SN - 0960-8524
VL - 121
SP - 480
EP - 483
JO - Bioresource Technology
JF - Bioresource Technology
ER -