Why do the abundances of ions generated by MALDI look thermally determined?

Yong Jin Bae, Joong Chul Choe, Jeong Hee Moon, Myung Soo Kim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

In a previous study (J. Mass Spectrom. 48, 299-305, 2013), we observed that the abundance of each ion in a matrix-assisted laser desorption ionization (MALDI) spectrum looked thermally determined. To find out the explanation for the phenomenon, we estimated the ionization efficiency and the reaction quotient (QA) for the autoprotolysis of matrix, M + M → [M + H] + + [M - H]-, from the temperature-controlled laser desorption ionization spectra of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB). We also evaluated the equilibrium constants (KA) for the autoprotolysis at various temperatures by quantum chemical calculation. Primary ion formation via various thermal models followed by autoprotolysis-recombination was compatible with the observations. The upper limit of the effective temperature of the plume where autoprotolysis- recombination occurs was estimated by equating QA with the calculated equilibrium constant. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1807-1815
Number of pages9
JournalJournal of the American Society for Mass Spectrometry
Volume24
Issue number11
DOIs
StatePublished - Nov 2013

Keywords

  • Autoprotolysis
  • MALDI
  • Primary ion formation
  • Recombination
  • Thermal determination

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