Wnt inhibitors enhance chondrogenesis of human mesenchymal stem cells in a long-term pellet culture

Gun Il Im, Jong Min Lee, Hye Joung Kim

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The long-term effects (~3 weeks) of two Wnt inhibitors (dickkopf [DKK]-1 and secreted frizzled-related protein [sFRP]-1), on the chondrogenic differentiation of human mesenchymal stem cells (hMSCs) was determined. Wnt inhibitors significantly increased the amount of glycosaminoglycan (GAG) in treated pellets (P < 0. 05). The gene expression of COL2A1 increased and COL1A1 decreased while the gene expression of SOX-9 and COL10A1 did not change significantly after three weeks of in vitro culture. The protein expression of type II collagen significantly increased (P < 0. 05) and that of type I collagen significantly decreased (P < 0. 05) while SOX-9 and type X collagen protein expression was unaffected. These findings suggest that Wnt inhibitors promote the chondrogenic differentiation of hMSCs when treated for three weeks.

Original languageEnglish
Pages (from-to)1061-1068
Number of pages8
JournalBiotechnology Letters
Volume33
Issue number5
DOIs
StatePublished - May 2011

Keywords

  • Chondrogenesis
  • Differentiation
  • Mesenchymal stem cell
  • Wnt signaling

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