Abstract
The long-term effects (~3 weeks) of two Wnt inhibitors (dickkopf [DKK]-1 and secreted frizzled-related protein [sFRP]-1), on the chondrogenic differentiation of human mesenchymal stem cells (hMSCs) was determined. Wnt inhibitors significantly increased the amount of glycosaminoglycan (GAG) in treated pellets (P < 0. 05). The gene expression of COL2A1 increased and COL1A1 decreased while the gene expression of SOX-9 and COL10A1 did not change significantly after three weeks of in vitro culture. The protein expression of type II collagen significantly increased (P < 0. 05) and that of type I collagen significantly decreased (P < 0. 05) while SOX-9 and type X collagen protein expression was unaffected. These findings suggest that Wnt inhibitors promote the chondrogenic differentiation of hMSCs when treated for three weeks.
Original language | English |
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Pages (from-to) | 1061-1068 |
Number of pages | 8 |
Journal | Biotechnology Letters |
Volume | 33 |
Issue number | 5 |
DOIs | |
State | Published - May 2011 |
Keywords
- Chondrogenesis
- Differentiation
- Mesenchymal stem cell
- Wnt signaling