Abstract
This study was performed to investigate the effect of Wnt inhibitors on chondrogenesis from adipose stem cells (ASCs). Two Wnt antagonists, secreted frizzled-related protein (sFRP)-1 and Dickkopf-related protein (DKK)-1, were treated on passage 3 ASCs in pellet culture. Cell viability was measured at various concentrations (2, 10, 50nM) of each inhibitor by MTT assay, and the inhibition of β-catenin was examined by western blot analysis. Chondrogenic differentiation was analyzed by glycosaminoglycan (GAG) assay and Safranin-O staining, and the gene and protein expression of chondrogenic markers by quantitative RT-PCR and Western blot analysis. The cell viability of ASCs was not significantly affected in the concentration of Wnt inhibitors we used. The expression of β-catenin decreased with the treatment of either inhibitor. There was a significant increase of proteoglycan, and the type II collagen gene and protein expression in ASCs treated with either sFRP-1 or DKK-1. The gene expression of type I collagen decreases while that of type X collagen increased with the treatment of either sFRP-1 or DKK-1. The overall results suggest that the Wnt inhibitiors enhanced chondrogenesis from ASCs.
Original language | English |
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Pages (from-to) | 610-615 |
Number of pages | 6 |
Journal | Tissue Engineering and Regenerative Medicine |
Volume | 7 |
Issue number | 5 |
State | Published - Nov 2010 |
Keywords
- Adipose stem cells (ASCs)
- Chondrogenesis
- DKK-1
- SFRP-1