Wnt inhibitors promote chondrogenesis from adipose stem cells

Insun Song, Gun Il Im

Research output: Contribution to journalArticlepeer-review

Abstract

This study was performed to investigate the effect of Wnt inhibitors on chondrogenesis from adipose stem cells (ASCs). Two Wnt antagonists, secreted frizzled-related protein (sFRP)-1 and Dickkopf-related protein (DKK)-1, were treated on passage 3 ASCs in pellet culture. Cell viability was measured at various concentrations (2, 10, 50nM) of each inhibitor by MTT assay, and the inhibition of β-catenin was examined by western blot analysis. Chondrogenic differentiation was analyzed by glycosaminoglycan (GAG) assay and Safranin-O staining, and the gene and protein expression of chondrogenic markers by quantitative RT-PCR and Western blot analysis. The cell viability of ASCs was not significantly affected in the concentration of Wnt inhibitors we used. The expression of β-catenin decreased with the treatment of either inhibitor. There was a significant increase of proteoglycan, and the type II collagen gene and protein expression in ASCs treated with either sFRP-1 or DKK-1. The gene expression of type I collagen decreases while that of type X collagen increased with the treatment of either sFRP-1 or DKK-1. The overall results suggest that the Wnt inhibitiors enhanced chondrogenesis from ASCs.

Original languageEnglish
Pages (from-to)610-615
Number of pages6
JournalTissue Engineering and Regenerative Medicine
Volume7
Issue number5
StatePublished - Nov 2010

Keywords

  • Adipose stem cells (ASCs)
  • Chondrogenesis
  • DKK-1
  • SFRP-1

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